CEN (European Committee for Standardisation
)-ISO (International Standardisation Organisation
)/TR 6579-3
Guidelines for serotyping of Salmonella are given in CEN-ISO/TR 6579-3:2014 ‘Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 3: Guidelines for serotyping of Salmonella spp.’
In this guidance document reference is made to the ‘White-Kauffmann-Le Minor scheme’, as described by Grimont and Weill (2007).
Grimont P .A.D., & W eill F.-X., 2007. Antigenic formulae of the Salmonella serovars, 9th edition. WHO (World Health Organization
)(World Health Organization ) Collaborating Centre for Reference and Research on Salmonella. Paris: Institut Pasteur. The scheme can be found at: https://www.pasteur.fr/sites/default/files/veng_0.pdf(externe link)(PDF)
Supplements to the White-Kauffmann-Le Minor scheme are published in Research in Microbiology, a publication of Institute Pasteur:
Guibourdenche, M., Roggentin, P. Mikoleit, M., Fields, P.I., Bockemühl, J., Grimont, P.A.D., Weill, F-X. Supplement 2003–2007 (No. 47) to the White–Kauffmann–Le Minor scheme. Research in Microbiology, 2010, 161, pp. 26–29. https://doi.org/10.1016/j.resmic.2009.10.002(externe link)
Issenhuth-Jeanjean, S., Roggentin, P., Mikoleit, M., Guibourdenche, M., de Pinna, E., Nair, S., I. Fields, P.I., Weill, F-X. Supplement 2008-2010 (no. 48) to the White-Kauffmann-Le Minor scheme. Research in Microbiology, 2014, 165, pp. 526-530. https://doi.org/10.1016/j.resmic.2014.07.004(externe link)
ISO 6579-4
Molecular identification and differentiation of the monophasic variant of Salmonella enterica subsp. enterica serovar Typhimurium (1,4,[5],12:i:-) lacking the second H phase H:1,2 is described in ISO 6579-4:2025 ‘Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 4: Identification of monophasic Salmonella Typhimurium (1,4,[5],12:i:-) by polymerase chain reaction (PCR).’
Background information on the validation of ISO 6579-4 can be found in:
Diddens, RE; van Hoek, AHAM; Malorny, B; Fischer, J; Mooijman, KA. Validation of ISO 6579-4 for identification of monophasic Salmonella Typhimurium (1,4,[5],12:i:-) by polymerase chain reaction (PCR). 2025. RIVM Report 2023-0430(PDF).
Alternative methods
For the validation of alternative methods for serotyping of Salmonella, the protocol of EN-ISO 16140-6 (‘Microbiology of the food chain – Method validation - Part 6: Protocol for the validation of alternative (proprietary) methods for microbiological confirmation and typing procedures’) shall be followed. For the use of alternative serotyping methods, similar rules apply as indicated for alternative detection methods [link].